Anti-ATF4 Antibody [S360A-24] (A304745)

Name: Anti-ATF4 Antibody [S360A-24]
Brand: Antibodies.com
SKU: A304745
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S360A-24] antibody to ATF4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ATF4 Antibody [S360A-24]

Description

Mouse monoclonal [S360A-24] antibody to ATF4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Rat

Cross Reactivity

This antibody does not cross-react with ATF5.

Immunogen

Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5

Host

Mouse

Clonality

Monoclonal

Clone ID

S360A-24

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activating transcription factor 4, ATF 4, ATF-4, ATF4 protein, ATF4_HUMAN, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4, Cyclic AMP response element binding protein 2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, DNA binding protein TAXREB67, DNA-binding protein TAXREB67, Tax Responsive Enhancer Element B67, Tax-responsive enhancer element-binding protein 67, TaxREB67, TXREB

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ATF4 Antibody [S360A-24] (A304745)

Western blot analysis of rat brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Anti-ATF4 Antibody [S360A-24] (A304745) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~39 kDa (isoform 2).

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Figure 2: Immunofluorescence - Anti-ATF4 Antibody [S360A-24] (A304745)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ATF4 Antibody [S360A-24] (A304745), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4 Antibody (D) Composite.

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Figure 3: Immunofluorescence - Anti-ATF4 Antibody [S360A-24] (A304745)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATF4 Antibody [S360A-24] (A304745), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATF4 Antibody. (D) Composite.

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