Anti-ATF3 Antibody (A88741)

Western blot analysis of extracts of 293T cells, using Anti-ATF3 Antibody (A88741) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of RAW264. 7 cells, using Anti-ATF3 Antibody (A88741) at 1:1,000 dilution. Raw264. 7 cells were treated by LPS (1 µg/ml) at 37°C for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ATF3 Antibody (A88741) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of U-2 OS cells using Anti-ATF3 Antibody (A88741) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).