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Anti-Atad5 Antibody (A326220)

Immunofluorescence - Anti-Atad5 Antibody (A326220) - Antibodies.com Flow Cytometry - Anti-Atad5 Antibody (A326220) - Antibodies.com
$448
Product Datasheet

Goat polyclonal antibody to Atad5 for ELISA, IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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+1 (314) 370-6046
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Name
Anti-Atad5 Antibody
Description
Goat polyclonal antibody to Atad5.
Applications
ELISA, IF, Flow Cytometry
Dilutions
ELISA: 1:32,000, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human Atad5.
Sequence
C-DNKIYPKNTKKKRVD
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ATAD5_HUMAN, ATPase family AAA domain containing 5, ATPase family AAA domain-containing protein 5, C17orf41, chromosome fragility associated gene 1, Chromosome fragility-associated gene 1 protein, ELG1, Enhanced level of genomic instability 1 homolog, FRAG1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-Atad5 Antibody (A326220) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-Atad5 Antibody (A326220)
Atad5 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Atad5 Antibody (A326220) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Flow Cytometry - Anti-Atad5 Antibody (A326220) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-Atad5 Antibody (A326220)
Atad5 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Atad5 Antibody (A326220) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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