Anti-ASK1 (phospho Ser83) Antibody (A10879)

Name: Anti-ASK1 (phospho Ser83) Antibody
Brand: Antibodies.com
SKU: A10879
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to ASK1 (phospho Ser83) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-ASK1 (phospho Ser83) Antibody

Description

Rabbit polyclonal antibody to ASK1 (phospho Ser83).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S83 of human MAP3K5 (NP_005914.1).

Sequence

GSSVG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

155 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis signal regulating kinase 1, Apoptosis signal-regulating kinase 1, ASK 1, ASK-1, M3K5, M3K5_HUMAN, MAP/ERK kinase kinase 5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen activated protein kinase kinase kinase 5, Mitogen-activated protein kinase kinase kinase 5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

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Figure 1: Western Blot - Anti-ASK1 (phospho Ser83) Antibody (A10879)

Western blot analysis of extracts of various cell lines, using Anti-ASK1 (phospho Ser83) Antibody (A10879) at 1:1,000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour, treated by TNF-alpha (20ng/ml) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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