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Anti-Asef Antibody (A83320)

Western Blot - Anti-Asef Antibody (A83320) - Antibodies.com Western Blot - Anti-Asef Antibody (A83320) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to Asef for ELISA and WB.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-Asef Antibody
Description
Goat polyclonal antibody to Asef.
Applications
ELISA, WB
Dilutions
ELISA: 1:32,000, WB: 2-4µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the N terminal region of human Asef.
Sequence
PWEEPAGEKPSCS
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
80 kDa
Predicted MW
79.1kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
APC stimulated guanine nucleotide exchange factor, APC stimulated guanine nucleotide exchange factor 1, ARHGEF 4, ARHGEF4, ASEF1, GEF, GEF 4, GEF4, KIAA1112, Rho guanine nucleotide exchange factor (GEF) 4, Rho guanine nucleotide exchange factor 4, STM 6, STM6
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Asef Antibody (A83320) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Asef Antibody (A83320)
Asef expression in Human Brain lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Asef Antibody (A83320) at 2µg/ml and detected by chemiluminescence.
Western Blot - Anti-Asef Antibody (A83320) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Asef Antibody (A83320)
Asef expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress Asef and incubated with Anti-Asef Antibody (A83320). Control: Mock-transfected HEK293 cell lysate incubated with Anti-Asef Antibody (A83320) (first lane).
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