Anti-ASCL4 Antibody (A88635)

Western blot analysis of extracts of various cell lines, using Anti-ASCL4 Antibody (A88635) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ASCL4 Antibody (A88635) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-ASCL4 Antibody (A88635) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-ASCL4 Antibody (A88635) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.