Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557)

Name: Anti-Aryl hydrocarbon Receptor Antibody [ARC53212]
Brand: Antibodies.com
SKU: A306557
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53212] antibody to Aryl hydrocarbon Receptor for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Cytoplasm Marker

Specifications

Name

Anti-Aryl hydrocarbon Receptor Antibody [ARC53212]

Description

Rabbit monoclonal [ARC53212] antibody to Aryl hydrocarbon Receptor.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR (NP_001612.1).

Sequence

LEQQQQHHQKQVVVEPQQQLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53212

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ah receptor, AhR, AHR_HUMAN, Aromatic hydrocarbon receptor, Aryl hydrocarbon receptor precursor, bHLHe76, Class E basic helix loop helix protein 76, Class E basic helix-loop-helix protein 76, HGNC:348

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557)

Western blot analysis of extracts of various cell lines, using Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Aryl hydrocarbon Receptor Antibody [ARC53212] (A306557) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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