Anti-Aromatase Antibody [ARC0635] (A307975)

Name: Anti-Aromatase Antibody [ARC0635]
Brand: Antibodies.com
SKU: A307975
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0635] antibody to Aromatase for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Aromatase Antibody [ARC0635]

Description

Rabbit monoclonal [ARC0635] antibody to Aromatase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aromatase (CYP19A1) (P11511).

Sequence

MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0635

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ARO1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Aromatase Antibody [ARC0635] (A307975)

Western blot analysis of extracts of U-87MG cells, using Anti-Aromatase Antibody [ARC0635] (A307975) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunohistochemistry - Anti-Aromatase Antibody [ARC0635] (A307975)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Aromatase Antibody [ARC0635] (A307975) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Aromatase Antibody [ARC0635] (A307975)

Immunofluorescence analysis of human placenta using Anti-Aromatase Antibody [ARC0635] (A307975) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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