Anti-ARK5 Antibody (A84498)

Name: Anti-ARK5 Antibody
Brand: Antibodies.com
SKU: A84498
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to ARK5 for ELISA, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-ARK5 Antibody

Description

Goat polyclonal antibody to ARK5.

Applications

ELISA, IF, Flow Cytometry

Dilutions

ELISA: 1:8,000, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human ARK5.

Sequence

C-QNRPRPQYLKRYRNR

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AMP activated protein kinase family member 5, AMPK related protein kinase 5, AMPK-related protein kinase 5, NUAK family SNF1 like kinase, NUAK family SNF1-like kinase 1, Nuak1, NUAK1_HUMAN, Omphalocele kinase 1, Probable serine/threonine protein kinase KIAA0537

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-ARK5 Antibody (A84498)

ARK5 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ARK5 Antibody (A84498) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-ARK5 Antibody (A84498)

ARK5 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ARK5 Antibody (A84498) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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