Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ARHGAP 25, Epididymis secretory protein Li 308, HEL S 308, KAIA0053, RHG25_HUMAN, Rho GTPase activating protein 25, Rho GTPase-activating protein 25, Rho type GTPase activating protein 25, Rho-type GTPase-activating protein 25
Figure 1: Western Blot - Anti-ARHGAP25 Antibody (A13391)
Western blot analysis of extracts of various cell lines, using Anti-ARHGAP25 Antibody (A13391) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-ARHGAP25 Antibody (A13391) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human gastric using Anti-ARHGAP25 Antibody (A13391) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of Jurkat cells using Anti-ARHGAP25 Antibody (A13391) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of L929 cells using Anti-ARHGAP25 Antibody (A13391) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
