Anti-Argonaute-2 Antibody [ARC0189] (A308366)

Name: Anti-Argonaute-2 Antibody [ARC0189]
Brand: Antibodies.com
SKU: A308366
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0189] antibody to Argonaute-2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Argonaute-2 Antibody [ARC0189]

Description

Rabbit monoclonal [ARC0189] antibody to Argonaute-2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Argonaute-2 (Q9UKV8).

Sequence

QRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0189

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ago 2, AGO2_HUMAN, Argonaute 2, argonaute 2, RISC catalytic component, Argonaute RISC catalytic component 2, Argonaute2, CTA-204B4.6, dAgo2, eIF 2C 2, eIF-2C 2, eIF2C 2, Eif2c2, Eukaryotic translation initiation factor 2C 2, Eukaryotic translation initiation factor 2C subunit 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, Protein argonaute-2, Protein slicer, Q10, Slicer protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Argonaute-2 Antibody [ARC0189] (A308366)

Western blot analysis of extracts of various cell lines, using Anti-Argonaute-2 Antibody [ARC0189] (A308366) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Argonaute-2 Antibody [ARC0189] (A308366)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Argonaute-2 Antibody [ARC0189] (A308366) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Argonaute-2 Antibody [ARC0189] (A308366)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Argonaute-2 Antibody [ARC0189] (A308366) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Argonaute-2 Antibody [ARC0189] (A308366)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Argonaute-2 Antibody [ARC0189] (A308366) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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