Mouse monoclonal [ARG1/1125] antibody to Arginase 1.
Specificity
This antibody recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 2-4 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 11-97, of human ARG1 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
ARG1/1125
Isotype
IgG3
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
35-38 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
A I, Al, ARG 1, arg1, ARGI1_HUMAN, Arginase liver, Arginase type I, Arginase, liver, Arginase-1, Arginase1, Liver Arginase, Liver type arginase, Liver-type arginase, Type I arginase
SDS-PAGE analysis of Anti-Arginase 1 Antibody [ARG1/1125] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Arginase 1 Antibody [ARG1/1125]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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