Anti-ARF5 Antibody [ARC1960] (A309207)

Name: Anti-ARF5 Antibody [ARC1960]
Brand: Antibodies.com
SKU: A309207
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1960] antibody to ARF5 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ARF5 Antibody [ARC1960]

Description

Rabbit monoclonal [ARC1960] antibody to ARF5.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 81-180 of human ARF5 (P84085).

Sequence

YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1960

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP ribosylation factor 5, ADP-ribosylation factor 5, ARF 5, ARF5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ARF5 Antibody [ARC1960] (A309207)

Western blot analysis of extracts of various cell lines, using Anti-ARF5 Antibody [ARC1960] (A309207) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-ARF5 Antibody [ARC1960] (A309207)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ARF5 Antibody [ARC1960] (A309207) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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