Anti-ARF1 Antibody [ARC1472] (A306088)

Name: Anti-ARF1 Antibody [ARC1472]
Brand: Antibodies.com
SKU: A306088
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1472] antibody to ARF1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ARF1 Antibody [ARC1472]

Description

Rabbit monoclonal [ARC1472] antibody to ARF1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human ARF1 (P84077).

Sequence

GQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1472

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

19 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ARF1 Antibody [ARC1472] (A306088)

Western blot analysis of extracts of various cell lines, using Anti-ARF1 Antibody [ARC1472] (A306088) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-ARF1 Antibody [ARC1472] (A306088)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ARF1 Antibody [ARC1472] (A306088) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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