Anti-ARF1 Antibody (A83143)

ARF1 expression in A431 (A) and HeLa (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody was performed with Anti-ARF1 Antibody (A83143) at 1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.

ARF1 expression in NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ARF1 Antibody (A83143) at 1µg/ml and detected by chemiluminescence.

ARF1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ARF1 Antibody (A83143) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.