Anti-Aquaporin 4 Antibody [ARC54345] (A80530)

Name: Anti-Aquaporin 4 Antibody [ARC54345]
Brand: Antibodies.com
SKU: A80530
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC54345] antibody to Aquaporin 4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Aquaporin 4 Antibody [ARC54345]

Description

Rabbit monoclonal [ARC54345] antibody to Aquaporin 4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 256-323 of human Aquaporin-4 (AQP4) (NP_001641.1).

Sequence

VEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC54345

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AQP 4, AQP-4, AQP4, AQP4_HUMAN, Aquaporin type 4, Aquaporin-4, Aquaporin4, HMIWC 2, HMIWC2, Mercurial insensitive water channel, Mercurial-insensitive water channel, MGC22454, MIWC, WCH 4, WCH4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Aquaporin 4 Antibody [ARC54345] (A80530)

Western blot analysis of extracts of Rat brain, using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Aquaporin 4 Antibody [ARC54345] (A80530)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Aquaporin 4 Antibody [ARC54345] (A80530)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Aquaporin 4 Antibody [ARC54345] (A80530)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Aquaporin 4 Antibody [ARC54345] (A80530) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.