Anti-Aquaporin 2 Antibody (A89116)

Name: Anti-Aquaporin 2 Antibody
Brand: Antibodies.com
SKU: A89116
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Aquaporin 2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Aquaporin 2 Antibody

Description

Rabbit polyclonal antibody to Aquaporin 2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human AQP2 (NP_000477.1).

Sequence

GAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADH water channel, AQP 2, AQP CD, AQP-2, AQP-CD, AQP2, AQP2_HUMAN, AQPCD, Aquaporin 2 collecting duct, Aquaporin CD, Aquaporin-2, Aquaporin-CD, Aquaporin2, Aquaporine 2, Collecting duct water channel protein, MGC34501, Water channel aquaporin 2, Water channel protein for renal collecting duct, WCH CD, WCH-CD, WCHCD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Aquaporin 2 Antibody (A89116)

Western blot analysis of extracts of 293T cells, using Anti-Aquaporin 2 Antibody (A89116) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Aquaporin 2 Antibody (A89116)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Aquaporin 2 Antibody (A89116)

Immunofluorescence analysis of rat kidney cells using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Aquaporin 2 Antibody (A89116)

Immunofluorescence analysis of mouse kidney cells using Anti-Aquaporin 2 Antibody (A89116) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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