Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
APO C3, Apo CIII, Apo-CIII, APOC 3, ApoC III, ApoC-III, APOC3, APOC3_HUMAN, ApoCIII, Apolipoprotein C III, Apolipoprotein C-III, Apolipoprotein C3, ApolipoproteinCIII, MGC150353
Figure 1: Western Blot - Anti-Apolipoprotein CIII Antibody (A91575)
Western blot analysis of extracts of various cell lines, using Anti-Apolipoprotein CIII Antibody (A91575) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse liver cells using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat liver cells using Anti-Apolipoprotein CIII Antibody (A91575) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).