Mouse monoclonal [G2] antibody to Apolipoprotein A I.
Specificity
This antibody recognises Apolipoprotein A-1 (also known as Apo-A1) , a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells. Mouse anti Human Apolipoprotein A1, clone G2 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or Albumin.
Applications
WB, ELISA, IHC-Fr
Dilutions
ELISA: 1:5,000, IHC-Fr: 1:40
Reactivity
Human
Immunogen
Native Apolipoprotein-A1 from human plasma.
Host
Mouse
Clonality
Monoclonal
Clone ID
G2
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of ascites.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in 0.01M Sodium Phosphate with 0.01M Sodium Borate, 0.15M Sodium Chloride, 1% Dextran, and 1% Mannitol.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human Apolipoprotein A1, clone G2 recognizes Apolipoprotein A-1 (also known as Apo-A1) , a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells.Mouse anti Human Apolipoprotein A1, clone G2 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or Albumin.