Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ANG 5, ANG-5, ANG5, Angiopoietin 5, Angiopoietin like 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, Angiopoietin-related protein 3, ANGL3_HUMAN, ANGPT5, ANL3, FHBL2, OTTHUMP00000010719, UNQ153/PRO179
Figure 1: Western Blot - Anti-ANGPTL3 Antibody [ARC1214] (A307333)
Western blot analysis of extracts of various cell lines, using Anti-ANGPTL3 Antibody [ARC1214] (A307333) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ANGPTL3 Antibody [ARC1214] (A307333) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat liver using Anti-ANGPTL3 Antibody [ARC1214] (A307333) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse liver using Anti-ANGPTL3 Antibody [ARC1214] (A307333) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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