Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Name: Anti-AMPK beta 1 (phospho Ser182) Antibody
Brand: Antibodies.com
SKU: A308809
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to AMPK beta 1 (phospho Ser182) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AMPK beta 1 (phospho Ser182) Antibody

Description

Rabbit polyclonal antibody to AMPK beta 1 (phospho Ser182).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S182 of human AMPKß1 (NP_006244.2).

Sequence

SSSPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

5'-AMP-activated protein kinase subunit beta-1, AMPK, AMPK subunit beta-1, AMPKb, PRKAB1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Western blot analysis of extracts of C2C12 cells, using Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809) at 1:1,000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-AMPK beta 1 (phospho Ser182) Antibody (A308809) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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