Anti-AMPK beta 1 Antibody (A92916)

Name: Anti-AMPK beta 1 Antibody
Brand: Antibodies.com
SKU: A92916
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to AMPK beta 1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-AMPK beta 1 Antibody

Description

Rabbit polyclonal antibody to AMPK beta 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKß1 (NP_006244.2).

Sequence

MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1300015D22Rik, 5''-AMP-activated protein kinase subunit beta-1, 5'-AMP-activated protein kinase beta-1 subunit, AAKB1_HUMAN, AMP-activated protein kinase beta subunit, AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1, AMP-activated, noncatalytic, beta-1, AMPK, AMPK beta 1 chain, AMPK subunit beta-1, AMPK-BETA-1, AMPKb, AU021155, E430008F22, HAMPKb, MGC17785, PRKAB1, Protein kinase AMP activated non catalytic subunit beta 1, protein kinase, AMP-activated, beta 1 non-catalytic subunit, protein kinase, AMP-activated, noncatalytic, beta-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-AMPK beta 1 Antibody (A92916)

Western blot analysis of extracts of various cell lines, using Anti-AMPK beta 1 Antibody (A92916) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-AMPK beta 1 Antibody (A92916)

Western blot analysis of extracts from normal (control) and AMPKbeta1 knockout (KO) HeLa cells, using Anti-AMPK beta 1 Antibody (A92916) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunofluorescence - Anti-AMPK beta 1 Antibody (A92916)

Immunofluorescence analysis of NIH-3T3 cells using Anti-AMPK beta 1 Antibody (A92916) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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