Anti-AMBRA1 Antibody (A13088)

Name: Anti-AMBRA1 Antibody
Brand: Antibodies.com
SKU: A13088
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to AMBRA1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-AMBRA1 Antibody

Description

Rabbit polyclonal antibody to AMBRA1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).

Sequence

GAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 - 150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activating molecule in beclin 1 regulated autophagy, Activating molecule in BECN1 regulated autophagy protein 1, Activating molecule in BECN1-regulated autophagy protein 1, AMRA1_HUMAN, Autophagy and beclin 1 regulator 1, DCAF3, FLJ20294, Hypothetical protein FLJ20294, Hypothetical protein LOC55626, KIAA1736, MGC33725, WDR94

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-AMBRA1 Antibody (A13088)

Western blot analysis of PANC-1, using Anti-AMBRA1 Antibody (A13088) at 1:800 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-AMBRA1 Antibody (A13088)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-AMBRA1 Antibody (A13088) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-AMBRA1 Antibody (A13088)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-AMBRA1 Antibody (A13088) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-AMBRA1 Antibody (A13088)

Immunofluorescence analysis of NIH/3T3 cells using Anti-AMBRA1 Antibody (A13088) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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