Anti-alpha Tubulin Antibody [YL1/2] (A282063)

Anti-alpha Tubulin Antibody [YL1/2]

Rat monoclonal [YL1/2] antibody to alpha Tubulin.

This antibody recognises the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987). Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

WB, IF, IP, IHC-Fr, ELISA, RIA

ELISA: 1:100 - 1:1,000, WB: 1:200 - 1:2,000

S. cerevisiae, Ashbya, Birds, Echinoderm, Human, Mouse, Dog, Rat, Pig, Drosophila, Plants, Amphibia, Saccharomyces, Pleurobrachia, Caenorhabditis, Dictyostelium discoideum, Xenopus, Pig-tailed macaque, Clytia sp., Arabidopsis, Strongylocentrotus purpuratus, Dendraster excentricus, Trypanosoma brucei, Potorous tridactylis, Bovine, Hemicentrotus pulcherrimus, Potato, Bombyx mori, Rhodnius prolixus, Beroe abyssicola, Candida sp.

This antibody does not cross react with Nephrotoma suturalis

Yeast tubulin.

Rat

Monoclonal

YL1/2

IgG2a

Unconjugated

1 mg/ml

Approximately 55 kDa in cell lysate from a wide range of species. Rat anti tubulin alpha antibody, clone YL1/2 is suitable for use as a Western Blotting loading control.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

Alpha-tubulin 1, ALS22, B ALPHA 1, bA408E5.3, H2 ALPHA, Hum a tub1, Hum a tub2, LIS3, MGC171407, MGC55332, TBA4A_HUMAN, Testis-specific alpha-tubulin, TUBA1, TUBA1A, tuba1l, TUBA2, TUBA3, TUBA3C, Tuba4a, Tubulin alpha, Tubulin alpha 1 chain, Tubulin alpha-1 chain, tubulin alpha-1B chain, Tubulin alpha-4A chain, Tubulin H2-alpha, Tubulin, alpha 1 (testis specific), tubulin, alpha 1, like, Tubulin, alpha 1a, Tubulin, alpha 3c, Tubulin, alpha 4a, Tubulin, alpha, testis-specific, Tubulin, alpha-1