Anti-alpha Tubulin Antibody [AMC0479] (A17303)

Name: Anti-alpha Tubulin Antibody [AMC0479]
Brand: Antibodies.com
SKU: A17303

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This product is discontinued

Anti-alpha Tubulin Antibody [AMC0479] (A17303) has been discontinued and is no longer available.

View all alpha Tubulin Antibodies.

Specifications

Name

Anti-alpha Tubulin Antibody [AMC0479]

Description

Mouse monoclonal [AMC0479] antibody to alpha Tubulin.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:5,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:100-1:200, IP: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human a-Tubulin.

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0479

Isotype

IgG1

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 1% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha-tubulin 1, Alpha-tubulin 3, Alpha-tubulin 3E, Testis-specific alpha-tubulin, TUBA1, TUBA1A, TUBA3, TUBA3E, TUBA4A, Tubulin alpha-1 chain, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin alpha-3E chain, Tubulin alpha-4A chain, Tubulin B-alpha-1, Tubulin H2-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-alpha Tubulin Antibody [AMC0479] (A17303)

Western blot analysis of extracts of various cell lines, using Anti-alpha Tubulin Antibody [AMC0479] (A17303) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-alpha Tubulin Antibody [AMC0479] (A17303)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-alpha Tubulin Antibody [AMC0479] (A17303)Immunohistochemistry analysis of paraffin-embedded mouse brain using alpha-Tubulin Mouse mAb (AC012) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-alpha Tubulin Antibody [AMC0479] (A17303)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-alpha Tubulin Antibody [AMC0479] (A17303)Immunohistochemistry analysis of paraffin-embedded mouse kidney using alpha-Tubulin Mouse mAb (AC012) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-alpha Tubulin Antibody [AMC0479] (A17303)

Confocal imaging of HeLa cells using Anti-alpha Tubulin Antibody [AMC0479] (A17303), at a dilution of 1:200, (green). The cells were counterstained with Anti-USO1 Antibody, at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.