Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Western blot analysis of extracts of various cell lines, using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of smooth muscle of human colon carcinoma using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse colon using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat colon using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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