Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024)

Name: Anti-alpha Sarcoglycan Antibody [ARC2280]
Brand: Antibodies.com
SKU: A309024
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2280] antibody to alpha Sarcoglycan for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-alpha Sarcoglycan Antibody [ARC2280]

Description

Rabbit monoclonal [ARC2280] antibody to alpha Sarcoglycan.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 288-387 of human alpha Sarcoglycan (SGCA) (SGCA) (Q16586).

Sequence

VDALVTLLVPLLVALLLTLLLAYVMCCRREGRLKRDLATSDIQMVHHCTIHGNTEELRQMAASREVPRPLSTLPMFNVHTGERLPPRVDSAQVPLILDQH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2280

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

50 DAG, 50 kDa dystrophin associated glycoprotein, 50 kDa dystrophin-associated glycoprotein, 50DAG, 50kD DAG, 59kDa, A2, adhalin, ADL, Alpha SG, Alpha-sarcoglycan, Alpha-SG, Asg, DAG2, DMDA2, Dystroglycan 2, Dystroglycan-2, LGMD2D, sarcoglycan, alpha (dystrophin-associated glycoprotein), SCARMD1, Sgca, SGCA_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024)

Western blot analysis of extracts of various cell lines, using Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024)

Immunofluorescence analysis of rat heart cells using Anti-alpha Sarcoglycan Antibody [ARC2280] (A309024) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).