Anti-alpha Sarcoglycan Antibody (A89891)

Name: Anti-alpha Sarcoglycan Antibody
Brand: Antibodies.com
SKU: A89891
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to alpha Sarcoglycan for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-alpha Sarcoglycan Antibody

Description

Rabbit polyclonal antibody to alpha Sarcoglycan.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human alpha Sarcoglycan (alpha Sarcoglycan (SGCA)) (NP_000014.1).

Sequence

ITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLPYQAEFLVRSHDAEEVLPSTPASRFLSALGGLWEPGELQLLNVTSALDRGGRVPLPIEGRKEGVYIKVGSASPFSTCLKMVASPDSHARCAQGQPPLLSCYDTLAPHFRVDWCNVTLVDKSVPEPADEVPTPGDGILEHDPFFCPPTEAPDRDFLVDA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

50 DAG, 50 kDa dystrophin associated glycoprotein, 50 kDa dystrophin-associated glycoprotein, 50DAG, 50kD DAG, 59kDa, A2, adhalin, ADL, Alpha SG, Alpha-sarcoglycan, Alpha-SG, Asg, DAG2, DMDA2, Dystroglycan 2, Dystroglycan-2, LGMD2D, sarcoglycan, alpha (dystrophin-associated glycoprotein), SCARMD1, Sgca, SGCA_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-alpha Sarcoglycan Antibody (A89891)

Western blot analysis of extracts of various cell lines, using Anti-alpha Sarcoglycan Antibody (A89891) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-alpha Sarcoglycan Antibody (A89891)

Immunofluorescence analysis of U2OS cells using Anti-alpha Sarcoglycan Antibody (A89891) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-alpha Sarcoglycan Antibody (A89891)

Immunofluorescence analysis of L929 cells using Anti-alpha Sarcoglycan Antibody (A89891) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-alpha Sarcoglycan Antibody (A89891)

Immunofluorescence analysis of C6 cells using Anti-alpha Sarcoglycan Antibody (A89891) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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