Anti-alpha Internexin Antibody [ARC2054] (A305822)

Name: Anti-alpha Internexin Antibody [ARC2054]
Brand: Antibodies.com
SKU: A305822
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2054] antibody to alpha Internexin for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Anti-alpha Internexin Antibody

Chicken polyclonal antibody to alpha Internexin for WB, ICC/IF and IHC.

Specifications

Name

Anti-alpha Internexin Antibody [ARC2054]

Description

Rabbit monoclonal [ARC2054] antibody to alpha Internexin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human Alpha Internexin (Q16352).

Sequence

EVAGYQDSIGQLENDLRNTKSEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTSGLSISGLNPLPNPSYLLPPRILSATTSKVSSTGLSLKKEE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2054

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 - 67 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

66 kDa neurofilament protein, AINX_HUMAN, Alpha Inx, Alpha-internexin, Alpha-Inx, INA, Internexin neuronal intermediate filament protein alpha, MGC12702, NEF 5, NEF5, Neurofilament 5, Neurofilament 5 (66kD), Neurofilament 66, Neurofilament 66 tax binding protein, Neurofilament-66, NF 66, NF-66, NF66, TXBP 1, TXBP1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-alpha Internexin Antibody [ARC2054] (A305822)

Western blot analysis of extracts of various cell lines, using Anti-alpha Internexin Antibody [ARC2054] (A305822) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-alpha Internexin Antibody [ARC2054] (A305822)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-alpha Internexin Antibody [ARC2054] (A305822) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-alpha Internexin Antibody [ARC2054] (A305822)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-alpha Internexin Antibody [ARC2054] (A305822) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-alpha Internexin Antibody [ARC2054] (A305822)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-alpha Internexin Antibody [ARC2054] (A305822) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.