Anti-alpha Internexin Antibody (A85446)

Mixed neuron glia cultures stained with Anti-Alpha-Internexin Antibody (red) and Anti-Coronin 1a Antibody (A85431 | green) - an excellent marker of microglia and lymphocytes. The Anti-Alpha-Internexin Antibody is an excellent marker of neuronal processes in these cultures.

Immunofluorescent analysis of a rat cerebellum section stained with Anti-alpha Internexin Antibody (A85446), at a dilution of 1:5,000, in red, and co-stained with Anti-MBP Antibody (A85322), at a dilution of 1:5,000, in green. The nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-alpha Internexin Antibody (A85446) selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the Anti-MBP Antibody (A85322) stains myelin sheathes around axons.

Western blot analysis of different tissue lysates using Anti-alpha Internexin Antibody (A85446), at a dilution of 1:10,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord and [7] pig spinal cord. The Anti-alpha Internexin Antibody (A85446) reveals the alpha Internexin protein with apparent molecular weight of 64-66 kDa, with some variability among different species.

Western blot of extract of rat brain stem crude extract stained with Anti-Alpha-Internexin Antibody - showing a single strong clean band at ~66kDa.