Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)

Name: Anti-Alpha B Crystallin Antibody [ARC1672]
Brand: Antibodies.com
SKU: A308835
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1672] antibody to Alpha B Crystallin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Alpha B Crystallin Antibody [ARC1672]

Description

Rabbit monoclonal [ARC1672] antibody to Alpha B Crystallin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 76-175 of human CRYAB (P02511).

Sequence

SVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1672

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AACRYA, Alpha crystallin B chain, Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, Cryab, CRYAB_HUMAN, Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)

Western blot analysis of extracts of various cell lines, using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Alpha B Crystallin Antibody [ARC1672] (A308835)

Immunofluorescence analysis of C6 cells using Anti-Alpha B Crystallin Antibody [ARC1672] (A308835) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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