Anti-alpha B Crystallin Antibody [1A7.D5] (A304737)

Name: Anti-alpha B Crystallin Antibody [1A7.D5]
Brand: Antibodies.com
SKU: A304737
Price: 450.0 USD
Availability: InStock

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$450

Mouse monoclonal [1A7.D5] antibody to alpha B Crystallin for WB, ELISA and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-alpha B Crystallin Antibody [1A7.D5]

Description

Mouse monoclonal [1A7.D5] antibody to alpha B Crystallin.

Specificity

Predicted mol. weight is ~21 kDa.

Applications

WB, ELISA, ICC/IF

Dilutions

WB: 1:2,000, ICC/IF: 1:100

Reactivity

Human, Bovine, Porcine, Rat

Cross Reactivity

This antibody does not cross-react with alpha A Crystallin, beta L Crystallin, beta H Crystallin, gamma Crystallin, HSP25, HSP27, or HSP47 proteins.

Immunogen

Native Human Alpha B Crystallin.

Host

Mouse

Clonality

Monoclonal

Clone ID

1A7.D5

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AACRYA, Alpha crystallin B chain, Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, Cryab, CRYAB_HUMAN, Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY REN 27, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-alpha B Crystallin Antibody [1A7.D5] (A304737)

Western blot analysis of Bovine cell lysates showing detection of Alpha B Crystallin protein using Anti-alpha B Crystallin Antibody [1A7.D5] (A304737) at 1:50 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-alpha B Crystallin Antibody [1A7.D5] (A304737)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-alpha B Crystallin Antibody [1A7.D5] (A304737), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha B Crystallin Antibody. (D) Composite.

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