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Anti-alpha 1 Fetoprotein Antibody [ARC54866] (A308567)

Western Blot - Anti-alpha 1 Fetoprotein Antibody [ARC54866] (A308567) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC54866] antibody to alpha 1 Fetoprotein for WB.

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Anti-alpha Fetoprotein Antibody [AFP-01]
Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.
Name
Anti-alpha 1 Fetoprotein Antibody [ARC54866]
Description
Rabbit monoclonal [ARC54866] antibody to alpha 1 Fetoprotein.
Applications
WB
Dilutions
WB: 1:1,000-1:5,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-609 of human Alpha-Fetoprotein (AFP) (NP_001125.1).
Sequence
RTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC54866
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
65 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA_HUMAN, Hereditary persistence of alpha fetoprotein, HPAFP
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-alpha 1 Fetoprotein Antibody [ARC54866] (A308567) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody [ARC54866] (A308567)
Western blot analysis of extracts of various cell lines, using Anti-alpha 1 Fetoprotein Antibody [ARC54866] (A308567) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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