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Anti-alpha 1 Fetoprotein Antibody (A81166)

Western Blot - Anti-alpha 1 Fetoprotein Antibody (A81166) - Antibodies.com Immunofluorescence - Anti-alpha 1 Fetoprotein Antibody (A81166) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to alpha 1 Fetoprotein for WB and ICC/IF.

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Highlighted products for Anti-alpha 1 Fetoprotein Antibody

Western Blot - Anti-alpha Fetoprotein Antibody [AFP-01] (A85532) - Antibodies.com
A85533
12 Citations
Anti-alpha Fetoprotein Antibody [AFP-01]
Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.
Western Blot - Anti-alpha Fetoprotein Antibody [AFP-01] (A85532) - Antibodies.com
A85533
12 Citations
Anti-alpha Fetoprotein Antibody [AFP-01]
Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.
Name
Anti-alpha 1 Fetoprotein Antibody
Description
Rabbit polyclonal antibody to alpha 1 Fetoprotein.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 395-609 of Alpha-Fetoprotein (AFP) (NP_001125.1).
Sequence
DKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
68 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA_HUMAN, Hereditary persistence of alpha fetoprotein, HPAFP
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-alpha 1 Fetoprotein Antibody (A81166) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody (A81166)
Western blot analysis of extracts of mouse liver, using Anti-alpha 1 Fetoprotein Antibody (A81166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunofluorescence - Anti-alpha 1 Fetoprotein Antibody (A81166) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-alpha 1 Fetoprotein Antibody (A81166)
Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control, (upper right) using Anti-alpha 1 Fetoprotein Antibody (A81166), at a dilution of 1:100, (Red). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-alpha 1 Fetoprotein Antibody (A81166)? Please let us know so that we can list the citation on this page.

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