Anti-ALK Antibody [ALK/1504] (A248625)

Anti-ALK Antibody [ALK/1504]

Mouse monoclonal [ALK/1504] antibody to ALK.

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

IHC-P: 1-2 µg/ml

Human

Recombinant fragment, around amino acids 200-335, of human ALK protein. The exact sequence is proprietary.

Mouse

Monoclonal

ALK/1504

IgG2b

Protein A/G chromatography.

200 µg/ml

80 kDa (hybrid) / 200 kDa (wild type)

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-ALK Antibody [ALK/1504] - BSA and Azide free (A251807).

ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246