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Anti-AlaRS Antibody (A393)

Detection of endogenous AlaRS protein in whole cell extracts by Western blotting with this antibody. HeLa and NIH3T3 lyates (10 µg). The anti-AlaRS antiserum was used at 1/300 dilution Immunofluorescence staining of AlaRS protein in HeLa cells by using anti-AlaRS antibody. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% TritonX100. The antibody was used at 1/100 dilution. As the second antibody, Alexa Fluor 488 conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. Nuclear DNA was stained with DAPI (left) and the merged image was shown in the center.
$480

Rabbit polyclonal antibody to AlaRS for WB and IF.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-AlaRS Antibody
Description
Rabbit polyclonal antibody to AlaRS.
Applications
WB, IF
Dilutions
WB: 1:100-1:1,000, IF: 1:100
Reactivity
Human, Hamster, Mouse
Immunogen
Recombinant hamster AlaRS protein (a.a. 695-969, GST tag).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Product Form
Liquid
Formulation
Supplied as antiserum with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AARS, AI316495, Alanine tRNA ligase, Alanine tRNA ligase 1, cytoplasmic, Alanine tRNA ligase cytoplasmic, Alanine--tRNA ligase, Alanyl tRNA synthetase, Alanyl tRNA synthetase cytoplasmic, Alanyl-tRNA synthetase, C76919, CMT2N, cytoplasmic, EC 6.1.1.7, MGC37368, Renal carcinoma antigen NY REN 42, Renal carcinoma antigen NY-REN-42, SYAC_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Detection of endogenous AlaRS protein in whole cell extracts by Western blotting with this antibody. HeLa and NIH3T3 lyates (10 µg). The anti-AlaRS antiserum was used at 1/300 dilutionEnlarge Image
Figure 1: Anti-AlaRS Antibody (A393)
Anti-AlaRS Antibody
Immunofluorescence staining of AlaRS protein in HeLa cells by using anti-AlaRS antibody. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% TritonX100. The antibody was used at 1/100 dilution. As the second antibody, Alexa Fluor 488 conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. Nuclear DNA was stained with DAPI (left) and the merged image was shown in the center.Enlarge Image
Figure 2: Immunofluorescence - Anti-AlaRS Antibody (A393)
Anti-AlaRS Antibody
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