Anti-Akt2 (phospho Ser474) Antibody (A94466)

Name: Anti-Akt2 (phospho Ser474) Antibody
Brand: Antibodies.com
SKU: A94466
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Akt2 (phospho Ser474) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Akt2 (phospho Ser474) Antibody

Description

Rabbit polyclonal antibody to Akt2 (phospho Ser474).

Specificity

This antibody detects endogenous levels of Akt2 only when phosphorylated at Ser474.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Akt2 around the phosphorylation site of Ser474 (amino acids 432-481).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

55kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30' using Anti-Akt2 (phospho Ser474) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Akt2 (phospho Ser474) Antibody (A94466)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Anti-Akt2 (phospho Ser474) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.