Anti-AKT2 Antibody (A90566)

Name: Anti-AKT2 Antibody
Brand: Antibodies.com
SKU: A90566
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to AKT2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-AKT2 Antibody

Description

Rabbit polyclonal antibody to AKT2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Akt2 (NP_001617.1).

Sequence

MRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AKT2_HUMAN, HIHGHH, murine thymoma viral (v-akt) homolog-2, PKB, PKB beta, PKBB, PKBBETA, PRKBB, Protein kinase Akt 2, Protein kinase Akt-2, Protein kinase B beta, rac protein kinase beta, RAC-BETA, RAC-beta serine/threonine-protein kinase, RAC-PK-beta, v akt murine thymoma viral oncogene homolog 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-AKT2 Antibody (A90566)

Western blot analysis of extracts of various cell lines, using Anti-AKT2 Antibody (A90566) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-AKT2 Antibody (A90566)

Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-AKT2 Antibody (A90566) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-AKT2 Antibody (A90566)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-AKT2 Antibody (A90566) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT2 Antibody (A90566)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-AKT2 Antibody (A90566) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.