Rabbit polyclonal antibody to AKT1 (phospho-Thr450)
Specificity
The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450) Antibody #11502 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-AKT1 Antibody [ARC51585] (A308667) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308667_1.jpg?profile=product_alternative)
![Western Blot - Anti-AKT1 Antibody [ARC51585] (A309277) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309277_1.jpg?profile=product_alternative)
![Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) - Antibodies.com](https://cdn.antibodies.com/image/catalog/80/A80471_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-AKT1 Antibody [AKT1/2784] (A248468) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248468_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-AKT1 Antibody [AKT1/2784] - BSA and Azide free (A251650) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251650_1.jpg?profile=product_alternative)
