This Akt1 (phospho Ser473) Cell Based ELISA Kit allows for the detection of Akt1 (phospho Ser473) and the effects that certain stimulation conditions have on Akt1 (phospho Ser473) expression in different cell lines. Qualitative determination of Akt1 (phospho Ser473) concentration is achieved by an indirect ELISA format. In essence, Akt1 (phospho Ser473) is captured by Anti-Akt1 (phospho Ser473) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-Akt1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Platform
Microplate
Detection Type
Fluorometric
Product Range
> 5000 Cells
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 4: Western Blot - Akt1 (phospho Ser473) Cell Based ELISA Kit (A103812)
The mouse monoclonal antibody to GAPDH is used as a positive control in Akt1 (phospho Ser473) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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