Anti-AKT1 Antibody [rAKT1/2491] - BSA and Azide free
Description
Recombinant mouse monoclonal [rAKT1/2491] antibody to AKT1.
Specificity
This antibody recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 85-189, of human AKT1 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
rAKT1/2491
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
62 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-AKT1 Antibody [rAKT1/2491] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma using Anti-AKT1 Antibody [rAKT1/2491].
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