Anti-AKT1 Antibody [ARC51585] (A309277)

Name: Anti-AKT1 Antibody [ARC51585]
Brand: Antibodies.com
SKU: A309277
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC51585] antibody to AKT1 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-AKT1 Antibody [ARC51585]

Description

Rabbit monoclonal [ARC51585] antibody to AKT1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human AKT1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51585

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-AKT1 Antibody [ARC51585] (A309277)

Western blot analysis of extracts of various cell lines, using Anti-AKT1 Antibody [ARC51585] (A309277) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-AKT1 Antibody [ARC51585] (A309277)

Western blot analysis of extracts from wild type(WT) and AKT1 knockout (KO) HeLa cells, using Anti-AKT1 Antibody [ARC51585] (A309277) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A309277)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-AKT1 Antibody [ARC51585] (A309277) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A309277)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-AKT1 Antibody [ARC51585] (A309277) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.