Anti-AKT1 Antibody (A285973)

Name: Anti-AKT1 Antibody
Brand: Antibodies.com
SKU: A285973
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to AKT1 for ELISA, WB and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-AKT1 Antibody

Description

Goat polyclonal antibody to AKT1.

Applications

ELISA, WB, IF

Dilutions

ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IF: 10µg/ml

Reactivity

Human

Cross Reactivity

This antibody is not expected to cross react with AKT2 but may recognize AKT1 in Rat and Canine.

Immunogen

Synthetic peptide corresponding to the internal region of human AKT1.

Sequence

C-QDVDQREAPLN

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

60 kDa

Predicted MW

55.7kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-AKT1 Antibody (A285973)

AKT1 expression in MCF7 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AKT1 Antibody (A285973) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-AKT1 Antibody (A285973)

AKT1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-AKT1 Antibody (A285973) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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