Recombinant rabbit monoclonal [RM251] antibody to AKT (phospho Ser473).
Specificity
This antibody recognizes AKT only when phosphorylated at Ser473.
Applications
WB, IHC
Dilutions
IHC: 1:200-1:500, WB: 1:1,000-1:2,000
Reactivity
Human
Cross Reactivity
This antibody does not cross-react with AKT without phosphorylation at Ser473. This antibody may react with samples from bovine, mouse, and rat, as predicted by immunogen homology.
Immunogen
Synthetic peptide corresponding to human AKT (phospho Ser473).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM251
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-AKT (phospho Ser473) Antibody [RM251] (A121248)
Western blot of lysates from 293 cells, either grown in medium with serum, serum starved, or insulin treated, using Anti-AKT (phospho Ser473) Antibody [RM251] at a 1:1,000 dilution or Anti-AKT1 Antibody [RM252] at a 1:1,000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-AKT (phospho Ser473) Antibody [RM251] at 1:400 dilution.
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