Anti-AHA1 Antibody [25F2.D9] (A304997)

Name: Anti-AHA1 Antibody [25F2.D9]
Brand: Antibodies.com
SKU: A304997
Price: 520.0 USD
Availability: InStock

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$520

Rat monoclonal [25F2.D9] antibody to AHA1 for WB, IHC, ICC/IF, IP and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-AHA1 Antibody [25F2.D9]

Description

Rat monoclonal [25F2.D9] antibody to AHA1.

Applications

WB, IHC, ICC/IF, IP, ELISA

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:1,000, IP: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Full Length Mouse Aha1 Protein.

Host

Rat

Clonality

Monoclonal

Clone ID

25F2.D9

Isotype

IgG2a

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1.2 mg/ml

Molecular Weight

~38 kDa (can run up to 45 kDa on SDS-PAGE)

Product Form

Liquid

Formulation

Supplied in 20mM Potassium Phosphate, pH 7.2, with 0.15M Sodium Chloride.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activator of 90 kDa heat shock protein ATPase homolog 1, Activator of heat shock 90kDa protein ATPase homolog 1, AHA 1, AHSA 1, Ahsa1, AHSA1_HUMAN, C14orf3, HSPC322, p38

Isotype Controls

Suitable Secondaries

See all Anti-Rat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-AHA1 Antibody [25F2.D9] (A304997)

Western blot analysis of human Cell lysates showing detection of Aha1 protein using Anti-AHA1 Antibody [25F2.D9] (A304997) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-AHA1 Antibody [25F2.D9] (A304997)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), using Anti-AHA1 Antibody [25F2.D9] (A304997), at 1:1,000. The secondary antibody used was FITC Goat Anti-Rat (green).

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Figure 3: Immunoprecipitation - Anti-AHA1 Antibody [25F2.D9] (A304997)

Immunoprecipitation analysis of human HeLa cells using Anti-AHA1 Antibody [25F2.D9] (A304997) at 1:1,000.

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Figure 4: Immunohistochemistry - Anti-AHA1 Antibody [25F2.D9] (A304997)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-AHA1 Antibody [25F2.D9] (A304997) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rat (green) at 1:50 for 1 hour at room temperature. Localization: Uppermost epidermis staining, and muscle.

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