Principle of Assay
Aflatoxin B1 Strip Test Kit (A327177) uses a competitive colloidal gold based format. The sample is added to the test well to allow the Aflatoxin B1 binding protein on the gold particles to engage with any Aflatoxin B1 present in the sample. Any gold particles that are not complexed with Aflatoxin B1 present in the sample will bind to the Aflatoxin B1 imprinted at the Test line (T-line), forming a signal (red line) at that position. If the Aflatoxin B1 binding protein on the gold particle has engaged with Aflatoxin B1 present in the sample sample, the gold particle will flow past the T-line and reach the Control line (C-line).