Anti-AF4 Antibody (A10032)

Name: Anti-AF4 Antibody
Brand: Antibodies.com
SKU: A10032

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This product is discontinued

Anti-AF4 Antibody (A10032) has been discontinued and is no longer available.

View all AF4 Antibodies.

Specifications

Name

Anti-AF4 Antibody

Description

Rabbit polyclonal antibody to AF4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).

Sequence

SQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

135 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AF4/FMR2 family member 1, AFF1, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2, PBM1, Protein AF-4, Protein FEL, Proto-oncogene AF4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-AF4 Antibody (A10032)

Western blot analysis of extracts of various cell lines, using Anti-AF4 Antibody (A10032) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.