Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Adipocyte enhancer binding protein 2, Adipocyte enhancer-binding protein 2, AE (adipocyte enhancer) binding protein 2, AE binding protein 2, AE-binding protein 2, AEBP 2, AEBP2_HUMAN, MGC17922, Zinc finger protein AEBP2
Figure 1: Western Blot - Anti-AEBP2 Antibody (A12114)
Western blot analysis of extracts of various cell lines, using Anti-AEBP2 Antibody (A12114) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AEBP2 Antibody (A12114) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-AEBP2 Antibody (A12114) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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