Anti-Adiponectin Antibody (A14049)

This product is discontinued

Anti-Adiponectin Antibody (A14049) has been discontinued and is no longer available.

View all Adiponectin Antibodies.

Specifications

Name

Anti-Adiponectin Antibody

Description

Rabbit polyclonal antibody to Adiponectin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-104 of human Adiponectin (NP_004788.1).

Sequence

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, ADIPOQ, Adipose most abundant gene transcript 1 protein, apM-1, APM1, GBP28, Gelatin-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Western Blot - Anti-Adiponectin Antibody (A14049)

Western blot analysis of extracts of various cell lines, using Anti-Adiponectin Antibody (A14049) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry - Anti-Adiponectin Antibody (A14049)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Adiponectin Antibody (A14049) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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