Anti-Adenylate Cyclase 3 Antibody (A85287)

Immunofluorescence of rat hippocampus stained with Anti-Adenylate Cyclase III Antibody (red) and Anti-MECP2 Antibody (green), DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The Anti-Adenylate Cyclase III Antibody reveals neuronal cilia while the Anti-MECP2 Antibody reveals the nuclei of certain neurons to a variable degree

Left: Confocal image of mixed rat neuron-glial cultures stained with Anti-Adenylate Cyclase III Antibody (red) and Anti-a-II-Spectrin Antibody (A85353 | green). Note the strong, clean and specific staining of neuronal cilia. Since a-II-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by the spectrin antibody. The Anti-a-II-Spectrin Antibody is also an excellent marker of neuronal plasma membranes. Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Anti-Adenylate Cyclase III Antibody (green) and Anti-Fox3/NeuN Antibody (A85404 | red), showing specific labeling of cilia next to the pyramidal neurons in CA1 hippocampus region, but not in other parts of the brain. Nuclei are labeled with DAPI (blue).

Western blot analysis of HEK293 cell lysates using Anti-Adenylate Cyclase 3 Antibody (A85287), at a dilution of 1:2,000, in green. The lanes contain samples of: [1] Protein standards, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130 kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250 kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The same blot was simultaneously probed with Anti-GAPDH Antibody (A85382), at a dilution of 1:5,000, in red, which reveals the single band at approximately 37 kDa in both transfected and non-transfected cells, acting as a positive loading control.

Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). Our antibody stains bands at about 200 kDa in olfactory epithelium, a tissue which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is also less heavily glycosylated, so that a much less prominent band is seen at about 160 kDa.